Forestry Engineering Journal Forestry Engineering Journal Лесотехнический журнал 2222-7962 55952 10.34220/issn.2222-7962/2022.4/2 Естественные науки и лес NATURAL SCIENCES AND FOREST Естественные науки и лес Effect of different led spectrum regimens on growth and development of Betula pubescens Ehrh. and Rubus idaeus L. in culture in vitro Воздействие светодиодных облучателей различного спектрального состава на рост и развитие Betula pubescens Ehrh. и Rubus idaeus L. в культуре in vitro Евлаков Петр Михаилович Evlakov P. M. peter.evlakov@yandex.ru

кандидат биологических наук;

candidate of sciences in biology;

 Гродецкая Татьяна Александровна Grodeckaya Tatyana Aleksandrovna tatyana.pokusina@yandex.ru Федорова Ольга Анатольевна Fedorova Olga A.

кандидат биологических наук;

candidate of sciences in biology;

 Шестаков Роман Анатольевич Shestakov Roman A. Баранов Олег Юрьевич Baranov Oleg Yur'evich

доктор биологических наук;

doctor of sciences in biology;

 ФГБОУ ВО Воронежский государственный лесотехнический университет им.Г.Ф. Морозова Россия ФГБОУ ВО Воронежский государственный лесотехнический университет им.Г.Ф. Морозова Russian Federation Воронежский государственный лесотехнический университет им. Г.Ф. Морозова Россия Воронежский государственный лесотехнический университет им. Г.Ф. Морозова Russian Federation Воронежский государственный лесотехнический университет имени Г.Ф. Морозова Воронеж Россия Воронежский государственный лесотехнический университет имени Г.Ф. Морозова Voronezh Russian Federation Воронежский государственный лесотехнический университет им. Г.Ф. Морозова Воронеж Россия Voronezh State University of Forestry and Technlogy named after G.F. Morozov Voronezh Russian Federation Национальная академия наук Беларуси Беларусь Национальная академия наук Беларуси Belarus 01 01 2023 01 01 2023 12 4 14 30 19 11 2022 18 12 2022 http://lestehjournal.ru/en/journal/2022/no-4-48/effect-different-led-spectrum-regimens-growth-and-development-betula-pubescens

Светодиоды (СД) показали высокую эффективность при выращивании растений как in vivo в теплицах, так и in vitro, в том числе и при клональном микроразмножении. Целью данного исследования было проанализировать влияние спектрального состава светодиодных облучателей c различной долей красного (КС) и синего (СС) света на морфогенез микрорастений ремонтантной формы малины обыкновенной (Rubus idaeus L.) сорта Геракл и селекционно-ценного сорта березы пушистой (Betula pubescens Ehrh.), являющейся объектом единого генетико-селекционного комплекса (ЕГСК), ранее отобранной по признакам засухоустойчивости. В варианте 1 эксперимента соотношение КС/СС было 80/20, в варианте 2 – 70/30, в контроле 50/50. СД варианта 1 в наибольшей степени способствовал улучшению морфометрических и анатомических характеристик у микроклонов малины, увеличивая высоту побегов, число листьев, плотность устьиц, высоту листового эпидермиса и мезофилла. В то же время повышение доли КС приводило к увеличению площади отдельных листьев, общей листовой поверхности и плотности устьиц у микроклонов березы, однако анатомические характеристики листа указывают на уменьшение высоты эпидермальных клеток, размера клеток мезофилла. Таким образом, СД варианта 1 можно рекомендовать для использования при клональном микроразмножении малины в теплицах, для оптимизации процессов роста и получения нормально сформированных растений, в то же время для березы требуется дополнительный подбор оптимальных условий спектрального освещения.

Light-emitting diodes (LEDs) have shown high efficiency in growing plants both in vivo in greenhouses and in vitro, including clonal micropropagation. The purpose of this study was to analyze the effect of the spectral composition of LED irradiators with different proportions of red (RL) and blue (BL) light on the morphogenesis of microplants of the remontant form of common raspberry (Rubus idaeus L.) cv. Hercules and the selection valuable cultivar of downy birch (Betula pubescens Ehrh.), which is the object of a unified genetic breeding complex (UGBC), previously selected on the basis of drought resistance. In the variant 1, the RL/BL ratio was 80/20%, in the variant 2 it was 70/30%, in the control it was 50/50%. The LED in variant 1 contributed to the greatest increase in morphometric and anatomical characteristics in raspberry microplants, increasing the height of shoots, the number of leaves, stomata density, the height of the leaf epidermis and mesophyll. At the same time, an increase in the proportion of RL/BL led to an increase in the leaf area, leaf surface, and stomatal density in birch microclones; however, the anatomical characteristics of the leaf indicate a decrease in the height of epidermal cells and the size of mesophyll cells. Thus, the LED of option 1 can be recommended for use in clonal micropropagation of raspberries in greenhouses, to optimize growth processes and obtain healthy, normally formed plants, while birch requires additional selection of optimal spectral illumination conditions.

 Betula pubescens Rubus idaeus in vitro светодиоды спектр света клональное микроразмножение мезоструктура листа морфогенез мезофилл Betula pubescens Rubus idaeus in vitro light emitting diodes light spectrum micropropagation leaf mesostructure morphogenesis mesophyll Исследование выполнено при финансовой поддержке ФГБОУ ВО «ВГЛТУ им. Г.Ф. Морозова» (внутривузовский грант № 6В/21). The study was carried out with the financial support of FSBEI HE «VSUFT named after G.F. Morozov» (intra-university grant No. 6B/21).

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