Acta biomedica scientifica Acta biomedica scientifica Acta biomedica scientifica 2541-9420 2587-9596 16551 10.12737/article_590823a565aa50.41723117 обзоры Review обзоры MODERN APPROACHES TO OXIDATIVE STRESS ESTIMATION, OR HOW TO MEASURE THE IMMEASURABLE СОВРЕМЕННЫЕ ПОДХОДЫ ПРИ АНАЛИЗЕ ОКИСЛИТЕЛЬНОГО СТРЕССА, ИЛИ КАК ИЗМЕРИТЬ НЕИЗМЕРИМОЕ Меньщикова Елена Брониславовна Menshchikova Elena Bronislavovna lemen@centercem.ru

доктор медицинских наук;

doctor of medical sciences;

 Зенков Николай Константинович Zenkov Nikolay Konstantinovich zenkovnk@mail.ru

доктор биологических наук;

doctor of sciences in biology;

 Научно-исследовательский институт экспериментальной и клинической медицины Новосибирск Россия Научно-исследовательский институт экспериментальной и клинической медицины Novosibirsk Russian Federation Научно-исследовательский институт экспериментальной и клинической медицины Новосибирск Россия Научно-исследовательский институт экспериментальной и клинической медицины Novosibirsk Russian Federation 1 3 174 180 https://zh-szf.ru/en/nauka/article/16551/view

Рассмотрены основные понятия, имеющие отношение к концепции окислительного стресса. Проанализированы существующие подходы и методы, используемые при его анализе, и отмечено, что окислительный стресс как таковой «измерить» нельзя. Возможно оценить: 1) выраженность и/или стационарную концентрацию тех или иных видов активированных кислородных метаболитов; 2) окислительное повреждение биомолекул; 3) содержание/активность антиоксидантов, – и для характеристики интенсивности окислительного стресса применять ту или иную комбинацию параметров.

Рассмотрены основные понятия, имеющие отношение к концепции окислительного стресса. Проанализированы существующие подходы и методы, используемые при его анализе, и отмечено, что окислительный стресс как таковой «измерить» нельзя. Возможно оценить: 1) выраженность и/или стационарную концентрацию тех или иных видов активированных кислородных метаболитов; 2) окислительное повреждение биомолекул; 3) содержание/активность антиоксидантов, – и для характеристики интенсивности окислительного стресса применять ту или иную комбинацию параметров.

 антиоксиданты активированные кислородные метаболиты окислительный стресс методы анализа

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