Foods and Raw Materials Foods and Raw Materials Foods and Raw Materials 2308-4057 2310-9599 6a84bb7894fbba14f785d856 10.21603/2308-4057-2019-2-264-273 Research Article Research Article Research Article Ultrasonic and microwave activation of raspberry extract: antioxidant and anti-carcinogenic properties Ultrasonic and microwave activation of raspberry extract: antioxidant and anti-carcinogenic properties https://orcid.org/0000-0002-9632-6296 Eremeeva Natalia B. Eremeeva Natalia B. rMWnatasha@rambler.ru https://orcid.org/0000-0002-0112-0085 Makarova Nadezhda V. Makarova Nadezhda V. Zhidkova Ekaterina M. Zhidkova Ekaterina M. Maximova Varvara P. Maximova Varvara P. Lesova Ekaterina A. Lesova Ekaterina A. Samara State Technical University Самара Россия Samara State Technical University Samara Russian Federation Samara State Technical University Самара Россия Samara State Technical University Samara Russian Federation N.N. Blokhin National Medical Research Centre of oncology Москва Россия N.N. Blokhin National Medical Research Centre of oncology Moscow Russian Federation N.N. Blokhin National Medical Research Centre of oncology Москва Россия N.N. Blokhin National Medical Research Centre of oncology Moscow Russian Federation N.N. Blokhin National Medical Research Centre of oncology Москва Россия N.N. Blokhin National Medical Research Centre of oncology Moscow Russian Federation 264 273 © Eremeeva N.B., Makarova N.V., Zhidkova E.M., Maximova V.P., Lesova E.A. © Eremeeva N.B., Makarova N.V., Zhidkova E.M., Maximova V.P., Lesova E.A. Eremeeva Natalia B., Makarova Nadezhda V., Zhidkova Ekaterina M., Maximova Varvara P., Lesova Ekaterina A. Eremeeva Natalia B., Makarova Nadezhda V., Zhidkova Ekaterina M., Maximova Varvara P., Lesova Ekaterina A. http://jfrm.ru/en/issues/1377/1401/

Safe and healthy nutrition has a beneficial effect on human well-being. Various foods, such as berries, are known to inhibit cancer-promoting pre-proliferative signals. Among European fruit and berry crops, raspberries demonstrate one with the widest ranges of biologically active substances. Extraction remains a reliable method of obtaining biologically active substances from plant materials. The research objective was to obtain a semi-finished raspberry product by using microwave and ultrasonic processing and to study its antioxidant, anti-carcinogenic, sensory, physico-chemical, and microbiological properties. The raspberry extracts were obtained by maceration, ultrasound treatment, and microwave processing. After that, the samples underwent a comparative analysis of their antioxidant properties. The ultrasonic method gave the best results. A set of experiments made it possible to define the optimal technological modes for the extraction process: ethanol = 50%, ultrasonic radiation = 35 kHz, temperature = 40 ± 5°C, time = 120 min, water ratio = 1:10. A set of experiments on cell cultures demonstrated that the raspberry extract was able to reduce the expression of the anti-inflammatory COX-2, iNOS, and IL-8 genes. Hense, we recommend further studies of the effect of the raspberry extract on the induced expression of COX-2, iNOS, and IL-8. In addition, its anticarcinogenic properties have to be studied in vivo.

Safe and healthy nutrition has a beneficial effect on human well-being. Various foods, such as berries, are known to inhibit cancer-promoting pre-proliferative signals. Among European fruit and berry crops, raspberries demonstrate one with the widest ranges of biologically active substances. Extraction remains a reliable method of obtaining biologically active substances from plant materials. The research objective was to obtain a semi-finished raspberry product by using microwave and ultrasonic processing and to study its antioxidant, anti-carcinogenic, sensory, physico-chemical, and microbiological properties. The raspberry extracts were obtained by maceration, ultrasound treatment, and microwave processing. After that, the samples underwent a comparative analysis of their antioxidant properties. The ultrasonic method gave the best results. A set of experiments made it possible to define the optimal technological modes for the extraction process: ethanol = 50%, ultrasonic radiation = 35 kHz, temperature = 40 ± 5°C, time = 120 min, water ratio = 1:10. A set of experiments on cell cultures demonstrated that the raspberry extract was able to reduce the expression of the anti-inflammatory COX-2, iNOS, and IL-8 genes. Hense, we recommend further studies of the effect of the raspberry extract on the induced expression of COX-2, iNOS, and IL-8. In addition, its anticarcinogenic properties have to be studied in vivo.

 Extraction of plant materials phenolic substances PRC-analysis expression of anti-inflammatory genes inhibition ultrasound microvaves Extraction of plant materials phenolic substances PRC-analysis expression of anti-inflammatory genes inhibition ultrasound microvaves

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