The effect of a biostimulator on the root formation of poplar cuttings Pyramidal-sedge Kamyshinsky
Rubrics: NATURAL SCIENCES AND FOREST
Authors:
1.
Voronezh State University of Forestry and Technologies named after G.F. Morozov
(Department of life safety and legal relations, professor)
from 01.01.2018 to 01.01.2019
Voronezh, Russian Federation
from 01.01.2018 to 01.01.2019
Voronezh, Russian Federation
2.
Voronezh State University of Forestry and Technologies named after G.F. Morozov
Russian Federation
Russian Federation
3.
FGBOU VO «Voronezhskiy gosudarstvennyy lesotehnicheskiy universitet imeni G.F. Morozova»
4.
Voronezh State University of Forestry and Technologies named after G.F. Morozov
Russian Federation
Russian Federation
5.
Voronezh State University of Forestry and Technologies named after G.F. Morozov
(kafedra mehanizacii lesnogo hozyaystva i proektirovaniya mashin, docent)
Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
6.
FGBOU VO «Voronezhskiy gosudarstvennyy lesotehnicheskiy universitet imeni G.F. Morozova»
Russian Federation
Russian Federation
Type:
Article
Pages:
from 5
to 18
Status:
Published
Received:
15.05.2025
Accepted:
15.09.2025
Published:
25.09.2025
Subject area:
UDC 338.439.223
Language:
Russian
Keywords:
amino acids, biostimulator, root formation of cuttings, Pyramid-sedge Kamyshinsky poplar, collagen
Funding:
this research received no external funding.
Abstract:
The problem of growing high-quality tree planting material is solved by using biologically active substances. Most poplars, including the Pyramid-sedge Kamyshinsky, are propagated by cuttings. But the weak root system of the pyramid-shaped poplars makes them vulnerable to drought, winds and mechanical damage. This paper presents the results of obtaining a biostimulator by fermenting collagen raw materials with actinomycete Streptomyces fradiae AC – 570 and evaluating its effect on the formation of adventitious roots in cuttings of the Pyramidal-sedge Kamyshinsky poplar. The amino acid composition of collagen hydrolysate was determined using liquid chromatography on a Shi-madzu LC-20 Prominence apparatus (Shimadzu, Japan). The degree of hydrolysis of collagen raw materials was as-sessed by amine nitrogen, using the Sorensen method. It was found that the optimal conditions for obtaining a bi-ostimulator are autoclaving of crushed raw materials with a 1:6 hydromodule for 20 minutes at a pressure of 0.1Mpa, followed by fermentation for 36 hours at a temperature of 28-30 0C, pH 6.5 – 7.0 and a concentration of culture of the microorganism of 10%. Processing of collagen raw materials under these conditions increased the degree of hydrol-ysis by 5.9 times. When observing these modes of collagen biodegradation, a high content of amino acids is provided, which allows it to be used as an amino acid stimulator of plants. The content of proline increased by 64.6%, and gly-cine by 7.5 times. Treatment of cuttings with a biostimulator increased the root mass by more than 3 times, and the leaf biomass of plants by 36.8%. The total weight of the cuttings of the prototypes exceeded the control variants by 1.9 times. The data obtained can be used to enhance the root formation of the cuttings of the Pyramid-sedge Ka-myshinsky poplar.
The problem of growing high-quality tree planting material is solved by using biologically active substances. Most poplars, including the Pyramid-sedge Kamyshinsky, are propagated by cuttings. But the weak root system of the pyramid-shaped poplars makes them vulnerable to drought, winds and mechanical damage. This paper presents the results of obtaining a biostimulator by fermenting collagen raw materials with actinomycete Streptomyces fradiae AC – 570 and evaluating its effect on the formation of adventitious roots in cuttings of the Pyramidal-sedge Kamyshinsky poplar. The amino acid composition of collagen hydrolysate was determined using liquid chromatography on a Shi-madzu LC-20 Prominence apparatus (Shimadzu, Japan). The degree of hydrolysis of collagen raw materials was as-sessed by amine nitrogen, using the Sorensen method. It was found that the optimal conditions for obtaining a bi-ostimulator are autoclaving of crushed raw materials with a 1:6 hydromodule for 20 minutes at a pressure of 0.1Mpa, followed by fermentation for 36 hours at a temperature of 28-30 0C, pH 6.5 – 7.0 and a concentration of culture of the microorganism of 10%. Processing of collagen raw materials under these conditions increased the degree of hydrol-ysis by 5.9 times. When observing these modes of collagen biodegradation, a high content of amino acids is provided, which allows it to be used as an amino acid stimulator of plants. The content of proline increased by 64.6%, and gly-cine by 7.5 times. Treatment of cuttings with a biostimulator increased the root mass by more than 3 times, and the leaf biomass of plants by 36.8%. The total weight of the cuttings of the prototypes exceeded the control variants by 1.9 times. The data obtained can be used to enhance the root formation of the cuttings of the Pyramid-sedge Ka-myshinsky poplar.
Keywords:
amino acids, biostimulator, root formation of cuttings, Pyramid-sedge Kamyshinsky poplar, collagen
amino acids, biostimulator, root formation of cuttings, Pyramid-sedge Kamyshinsky poplar, collagen
