Human glutathione-S-transferases play an important role in phase II detoxification process. But poly-morphism in the GSTP1 gene has not been studied in certain populations.Aim: to determine the distribution of allele and genotype frequencies of GSTP1 gene in teenagers from Buryat ethnic group.Materials and methods. Blood samples were obtained from 55 teenagers from Buryat ethnic group. There were 28 males and 27 females with an age of 14.05±0.99 years over the range of 13 to 16. DNA was isolated from blood samples. The polymerase chain reaction was used to amplify A313G and C341T markers of the GSTP1 gene. Chi-square testing was used to evaluate the significant difference of the GSTP1 genotype frequencies between observed and expected values.Results. Allele and genotype frequencies of A313G and C341T markers GSTP1 were determined in teenagers from Buryat ethnic group. The study showed that the frequencies of A and G alleles at the A313G marker were 0.809 and 0.191 while those of C and T alleles at the C341T marker were 0.973 and 0.027, respectively. The distribution of the genotype frequencies at the A313G marker were consistent with expected in a Hardy–Weinberg equilibrium (χ2=0 .77; d.f.=1; p>0.05). However, the distribution of the genotype frequencies at the C341T marker were not consistent with expected in a Hardy – Weinberg equilibrium (χ2= 0.043; d.f.= 1; p<0.05). It was because the homozygous of T allele was not found in the ethnic group of Buryat.
gene polymorphism, ethnic groups, teenagers
1. BaranovVS (2009). The genetic passport - the basis of individual and predictive medicine [Geneticheskiy pasport- osnova individual’noy i prediktivnoy med-itsiny],527.
2. DarenskayaMA (2012). Ethnic and regional aspects of human pathological processes [Etnicheskie i regional’nye aspekty patologicheskikh protsessov u cheloveka]. Bul-leten’ Vostocno-Sibirskogo naucnogo centra, (2), 145-151.
3. KalininaEV, ChernovNN, NovichkovaMD (2014). The role of glutathione, glutathione and glutaredoxin in the regulation of redox-dependent processes [Rol’ gluta-tiona, glutationtransferazy i glutaredoksina v regulyatsii redoks-zavisimykh protsessov]. Uspekhi biologicheskoy khimii, (54), 299-348.
4. KolesnikovaLI, KurashovaNA, GrebenkinaLA, Dol-gihMI, LabyginaAV, SuturinaLV, DashievBG, DarzhaevZJ(2011). Features of oxidative stress in men of different ethnic groups, obesity and infertility [Osobennosti okis-litel’nogo stressa u muzhchin raznykh etnicheskikh grupp s ozhireni-em i besplodiem]. Zdorov’e. Meditsinskaya ekologiya. Nauka, 44(1), 38-41.
5. KolesnikovaLI, GrebenkinaLA, DarenskajaMA, Vlas-ovBJ (2012). Oxidative stress as a nonspecific pathoge-netic link of reproductive disorders (review) [Okislitel’nyy stress kak nespetsificheskoe patogeneticheskoe zveno reproduktivnykh narusheniy (obzor)]. Sibirskiy nauchnyy meditsinskiy zhurnal, 32(1), 58-66.
6. KulinskiyVI (1999). Neutralization of xenobiotics [Obezvrezhivanie ksenobiotikov]. Sorosovskiy obrazova-tel’nyy zhurnal, (1), 8-12.
7. LimborskaSA, KhusnutdinovaEK, BalanovskayaEV (2012). Ethnogenomics and genogeography of peoples of Eastern Europe [Etnogenomika i genogeografiya narodov Vostochnoy Evropy], 261.
8. MusinAG, KhaziyevAV, NigmatullinaAE (2014). Poly-morphism of genes of xenobiotic detoxification system, its role in the biotransformation of drugs [Polimorfizm genov sistemy detoksikatsii ksenobiotikov, ego rol’ v bio-transformatsii lekarstvennykh preparatov]. Meditsinskiy vestnik Bashkortostana, 9(2), 211-216.
9. PoltanovaAA, AgarkovaLA, BukharinIY (2013). Func-tional differences are genetically determined variants of xenobiotic detoxification system in the formation process of gestational complications [Funktsional’nye razlichiya geneticheski determinirovannykh variantov sistemy detoksikatsii ksenobiotikov v formirovanii oslozhneniy gestatsionnogo protsessa]. Sovremennye problemy nauki i obrazovaniya, (6), 9.
10. SpitsinVA (2008). Environmental human genetics [Ekologicheskaya genetika cheloveka], 503.
11. ChernyakYI, GrassmanDA, KolesnikovSI (2007). The impact of persistent organic pollutants exposure on xenobiotics biotransformation [Vliyanie stoykikh organicheskikh zagryazniteley na biotrasformatsiyu ksenobiotikov],134.
12. CheninVA, LabyginaAV, UrybinIY, SuturinaLV, Laza-revLM, YermolovEV, BelyaevaEV (2009). Polymorphism of genes of detoxification system in women with endometrio-sis and infertility [Polimorfizm genov sistemy detoksikatsii u zhenshchin s genital’nym endometriozom i besplodiem]. Bulleten’ Vostocno-Sibirskogo naucnogo centra, (2), 74-75
13. HayesJD, FlanaganJU, JowseyIR (2005). Glutathi-one transferases. Ann. Rev. Pharmacol. Toxicol., (45), 51-88
14. IshiiT, MatsuseT, TeramotoS (1999). Glutathione S-transferase P1 (GSTP1) polymorphism in patients with chronic obstructive pulmonary disease. Thorax, (54), 693-696
15. KangsadalampaiS, GamnaraiP, RojpibulstitP(2008). Gene frequencies of the polimorphic human glu-tathione S-transferase class Pi: are they race-dependent? Thammasat Int. J. Sci. Tech., 1(1), 17-21.
16. TewKD, TownsendDM (2012). Glutathi-one-S-transferases as determinants of cell survival and death. Antioxidants & Redox Signaling, (17), 1728-1737
17. Van LieshoutE, KnapenM, LangeW (1998). Lo-calization of glutathione-S-transferase α and π in human embryonic tissues at 8 weeks gestational age. Human Reproduction, (13), 1380-1386.
18. WuB, DongD (2012). Human cytosolic glutathi-one transferases: structure, function, and drug discovery. Trends in Pharmacological Sciences, (33), 656-668
