Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
All-Russian research institute of forest genetics, selection and biotechnology (Director)
Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
The article discusses the use of microsatellite (SSR) markers for the estimation of genetic resources of birch. It is shown that DNA analysis using 12 microsatellites is most suitable for intraspecific differentiation of selectively valua-ble genotypes of this tree species. The genetic polymorphism in the studied birch genotypes was determined from the loci examined by SSR. Multilocus genetic passports were prepared for 31 samples of birch, which allowed (based on the presence (1) / absence (0) of 36 alleles) to identify the investigated samples.
birch, Betula, genotyping, microsatellites, SSR, polymorphism, PCR, passportization.
Введение. В настоящее время методы молекулярной генетики всё шире применяются в лесном хозяйстве для оценки и мониторинга состояния лесных генетических ресурсов, управления процессами лесовосстановления, фитосанитарного мониторинга лесных насаждений и питомников, получения селекционного посадочного материала. Одной из основных задач лесного хозяйства является лесовосстановление и лесоразведение. Быстрое и качественное лесовосстановление возможно только при выполнении ряда условий, таких как использование на плантациях улучшенного посадочного материала и его надежная идентификация, в том числе и с применением молекулярно-генетических методов.
1. Zienkiewicz E. Genome fingerprinting by simple sequence repeats (SSR)-anchored polymerase chain reaction amplification / E. Zienkiewicz, A. Rafalski, V. Labuda // Genomics. - № 20. - P. 176-183.
2. Microsatellite sequences: a new generation of molecular markers for forest genetics / F. Lefort, C. Echt, R. Streiff, G. G. Vendramin // Forest Genetics. - 1999. - № 6 (1). - P. 15-20.
3. Järvinen P. Nucleotide variation of birch (Betula L.) species: population structure and phylogenetic relationships: PhD diss. / P. Järvinen. - Joensuu, 2004. - 138 p.
4. Izucheniye geneticheskoy struktury populyatsiy karelskoy berozy v Respublike Karelii s pomoshchyu mikrosatellitnogo analiza / L. V. Vetchinnikova, A. F. Titov, L. V. Topchiyeva, N. L. Rendakov // Problemy obyektov lesnoy nauki: sovremennoe sostoyanie i perspektivy. - Voronezh, 2014. - S. 10-12.
5. Otsenka geneticheskogo raznoobraziya populyatsiy karelskoy berezy v Karelii s pomoshchyu mikrosatellitnykh markerov / L. V. Vetchinnikova, A. F. Titov, L. V. Topchiyeva, N. L. Rendakov // Ekologicheskaya genetika. - 2012. - T. 10. - № 1. - S. 34-37.
6. Isakov I. Yu. Genotipirovanie selektsionnykh form listvennykh drevesnykh rasteniy s ispolzovaniem baz dannykh sikvensov DNK / I. Yu. Isakov // Problemy obyektov lesnoy nauki: sovremennoe sostoyanie i perspektivy. - Voronezh, 2014. - S. 42-43.
7. Population history, genetic variation and conservation status of the endangered birch species Betula nana L. in Poland / K. A. Jadwiszczak, D. Drzymulska, A. Banaszek, P. Jadwiszczak // Silva Fennica. - 2012. - № 46 (4). - P. 465-477.
8. Genetic diversity and genetic structure of adult and buried seed populations of Betula maximowicziana in mixed and post-fire stands / K. Uchiyama, S. Goto, Y. Tsuda, Y. Takahashi, Y. Ide // Forest Ecology and Management. - 2006. - № 237. - P. 119-126.
9. Kulju K. K. M. Twenty-three microsatellite primer pairs for Betula pendula (Betulaceae) / K. K. M. Kulju, M. Pekkinen, S. Varvio // Molecular Ecology Notes. - 2004. - № 4. - P. 471-473.
10. Isolation and characterization of microsatellite markers in the tetraploid birch, Betula pubescens ssp. tortuosa / C. Truong, A. E. Palmé, F. Felber, Y. Naciri-Graven // Molecular Ecology Notes. - 2005. - № 5. - P. 96-98.
11. Isolation and characterization of 19 microsatellite markers in a tropical and warm subtropical birch, Betula alnoides Buch.-Ham. ex D. Don / J. J. Guo, J. Zeng, S. L. Zhou, Z. G. Zhao // Molecular Ecology Resources. - 2008. - № 8 (4). - P. 895-897.
12. Gürcan K. Transferability of microsatellite markers in the Betulaceae / K. Gürcan, Sh. A. Mehlenbacher // J. Amer. Soc. Hort. Sci. - 2010. - № 135 (2). - P. 159-173.
13. Cross-species amplification of Betula pendula Roth simple sequence repeat markers in Alnus species / A. Zhuk, I. Veinberga, M. Daugavietis, D. Ruņģis // Baltic Forestry. - 2008. - № 14 (2). - P. 116-121.
14. Isakov Yu. N. Integratsionno-epigeneticheskoye proiskhozhdeniye karelskoy berozy: gipoteza i fakty / Yu. N. Isakov, N. M. Soustova, I. Yu. Isakov // Strukturnye i funktsionalnye otkloneniya ot normalnogo rosta i razvitiya rasteniy pod vozdeystviyem faktorov sredy. - Petrozavodsk, 2011. - S. 98-103.
15. Palmé A. Evolution history and chloroplast DNA variation in three plant genera: Betula, Corylus and Salix. The impact of post-glacial colonisation and hybridisation: Comprehensive summaries of dokt. diss. / A. Palmé. - Uppsala, 2003. - 59 p.
16. Linkage map of birch, Betula pendula Roth, based on microsatellites and amplified fragment length polymorphisms / M. Pekkinen, S. Varvio, K. K. M. Kulju, H. Kärkkäinen, S. Smolander, A. Viherä-Aarnio, V. Koski, M. J. Sillanpää // Genome. - 2005. - № 48. - P. 619-625.
17. Doyle J. J. A rapid DNA isolation procedure for small quantities of fresh leaf tissue / J. J. Doyle, J. L. Doyle // Phytochem Bull. - 1987. - № 19. - P. 11-15.
