Comparison of the Therapeutic Potential of Rat and Human Mesenchymal Stromal Cells and Their Conditioned Media in Local Radiation Lesions

A. Rastorgueva; T. Astrelina; V. Brunchukov; D. Usupzhanova; I. V. Kobzeva; V. Nikitina; S. Lischuk; E. Dubova; K. Pavlov; V. Brumberg; A. Samoylov

doi:10.12737/1024-6177-2021-66-4-5-12

UDC 61 CSCSTI 76.33 Russian Classification of Professions by Education 31.06.2001 Russian Library and Bibliographic Classification 534 Russian Trade and Bibliographic Classification 5712 CSCSTI 76.03 Russian Classification of Professions by Education 31.08.08 Russian Classification of Professions by Education 32.08.12 Russian Classification of Professions by Education 14.04.02 Russian Library and Bibliographic Classification 51 Russian Trade and Bibliographic Classification 5734 Russian Trade and Bibliographic Classification 6212 Russian Trade and Bibliographic Classification 5708

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COMPARISON OF THE THERAPEUTIC POTENTIAL OF RAT AND HUMAN MESENCHYMAL STROMAL CELLS AND THEIR CONDITIONED MEDIA IN LOCAL RADIATION LESIONS Comparison of the Therapeutic Potential of Rat and Human Mesenchymal Stromal Cells and Their Conditioned Media in Local Radiation Lesions

АА Аа

Published in Medical Radiology and radiation safety · Volume 66, Issue 4 · Pages 5–12 · Rubric: Radiation biology

DOI: https://doi.org/10.12737/1024-6177-2021-66-4-5-12

Received: 13.09.2021 Accepted: 24.03.2024

Authors

A. Rastorgueva

T. Astrelina

V. Brunchukov

D. Usupzhanova

et al.

I. V. Kobzeva

V. Nikitina

S. Lischuk

E. Dubova

K. Pavlov

V. Brumberg

A. Samoylov

A. Rastorgueva

A.I. Burnasyan Federal Medical Biophysical Center of FMBA Junior Researcher · Russian Federation · Moscow

T. Astrelina

Academic degree: doctor of medical sciences

A.I. Burnasyan Federal Medical Biophysical Center (FMBC) FMBA Head of Center for Biomedical Technologies · Russian Federation · Moscow

V. Brunchukov

A.I. Burnasyan Federal Medical Biophysical Center (FMBC) FMBA Junior Researcher · Russian Federation · Moscow

D. Usupzhanova

A.I. Burnasyan Federal Medical Biophysical Center (FMBC) FMBA Junior Researcher · Russian Federation · Moscow

I. V. Kobzeva

Academic degree: candidate of medical sciences

A.I. Burnasyan Federal Medical Biophysical Center (FMBC) FMBA Head of Center for Biomedical Technologies Cryobank · Russian Federation · Moscow

V. Nikitina

Academic degree: candidate of medical sciences

A.I. Burnasyan Federal Medical Biophysical Center (FMBC) FMBA Leading Researcher · Russian Federation · Moscow

S. Lischuk

Academic degree: candidate of medical sciences

A.I. Burnasyan Federal Medical Biophysical Center of FMBA Head of Department · Russian Federation · Moscow

E. Dubova

Academic degree: candidate of medical sciences ; doctor of medical sciences

A.I. Burnasyan Federal Medical Biophysical Center of FMBA Doctor Pathologist · Russian Federation · Moscow

K. Pavlov

Academic degree: candidate of medical sciences

A.I. Burnasyan Federal Medical Biophysical Center of FMBA Doctor Pathologist · Russian Federation · Moscow

V. Brumberg

A.I. Burnasyan Federal Medical Biophysical Center of FMBA Biologist · Russian Federation · Moscow

A. Samoylov

Academic degree: doctor of medical sciences

Science rang: professor author.academy_titles.32

A.I. Burnasyan Federal Medical Biophysical Center of FMBA Director General · Russian Federation · Moscow

ABSTRACT

Background: To compare the results of the use of mesenchymal stromal cells (MSCs) of human gingival mucosa and MSCs of rat gingival mucosa, their conditioned media, and to evaluate their effect on tissue regeneration in local radiation injury (LRI). Material and methods: The study included 120 white male Wistar rats weighing 210 ± 30 g at the age of 8–12 weeks, randomized into 6 groups (20 animals each): control (C), animals did not receive therapy; control with the introduction of culture medium concentrate (CM) three times for 1, 14, 21 days; administration of human gingival mucosa MSCs (HM) at a dose of 2 million per 1 kg three times for 1, 14, 21 days; administration of human gingival mucosa MSCS conditioned medium concentrate (HMCM) at a calculated dose of 2 million cells per 1 kg three times for 1, 14, 21 days; administration of rat gingival mucosal MSCs (RM) at a dose of 2 million cells per 1 kg three times for 1, 14, 21 days; administration of rat gingival mucosal MSCS (RMCM) conditioned medium concentrate at a calculated dose of 2 million cells per 1 kg three times for 1, 14, 21 days. Each laboratory animal was observed 17 times: on 1, 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 77, 84, 91, 98, 105, 112 day after the burn simulation. Histological (hematoxylin-eosin staining) and immunohistochemical (CD31, CD68, VEGF, PGP 9.5, MMP2,9, Collag 1, TIMP 2) studies were performed. LRI was modeled on an X-ray machine at a dose of 110 Gy. MSCs were cultured according to the standard method up to 3–5 passages, the conditioned medium was taken and concentrated 10 times. The immunophenotype of MSCs (CD34, CD45, CD90, CD105, CD73, HLA-DR) and viability (7‑ADD) were determined by flow cytofluorimetry. Results: In a comparative analysis with the control group (C), starting from the 42nd day of the study, a tendency to reduce the area of skin ulcers in animals in all groups was observed, despite the fact that not all days had statistically significant differences. On day 112th, complete healing of skin ulcers in the CM group was observed in 40 % of animals in the HM group – in 60 %, in the HMCM group – in 20 % of animals, in the RMCM group–20 %, and in the C and RM groups there were no animals with a prolonged wound defect. Positive expression of the VEGF marker was observed in groups C and CM on the 28th day and in experimental groups (HM, HMCM, RM, RMCM) on the 112th day. A statistically significant increase in the CD68 marker was observed in groups C, RM, and RMCM, while the remaining groups showed a decrease in the number of macrophages.

KEYWORDS

mesenchymal stromal cells, local radiation lesions, conditioned medium, cell technologies, X-ray radiation, skin

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GOST

Rastorgueva A. A., Astrelina T. A., Brunchukov V. A., Usupzhanova D. Y., Kobzeva I. V., Nikitina V. A., Lischuk S. V., Dubova E. A., Pavlov K. A., Brumberg V. A., Samoylov A. S. Comparison of the Therapeutic Potential of Rat and Human Mesenchymal Stromal Cells and Their Conditioned Media in Local Radiation Lesions // Medical Radiology and radiation safety. 2021. no. 4. pp. 5-12. DOI: https://doi.org/10.12737/1024-6177-2021-66-4-5-12. (Date of access 12.10.2026).

MLA

A. Rastorgueva, Astrelina T. A., Brunchukov V. A., Usupzhanova D. Y., Kobzeva I. V., Nikitina V. A., Lischuk S. V., Dubova E. A., Pavlov K. A., Brumberg V. A., Samoylov A. S.. "Comparison of the Therapeutic Potential of Rat and Human Mesenchymal Stromal Cells and Their Conditioned Media in Local Radiation Lesions." Medical Radiology and radiation safety 66.4 (2021): 5-12. DOI: https://doi.org/10.12737/1024-6177-2021-66-4-5-12.

APA

Rastorgueva A. A., Astrelina T. A., Brunchukov V. A., Usupzhanova D. Y., Kobzeva I. V., Nikitina V. A., Lischuk S. V., Dubova E. A., Pavlov K. A., Brumberg V. A., Samoylov A. S. (2021). Comparison of the Therapeutic Potential of Rat and Human Mesenchymal Stromal Cells and Their Conditioned Media in Local Radiation Lesions. Medical Radiology and radiation safety, 66(4), 5-12. https://doi.org/10.12737/1024-6177-2021-66-4-5-12

Chicago

A. Rastorgueva, Astrelina T. A., Brunchukov V. A., Usupzhanova D. Y., Kobzeva I. V., Nikitina V. A., Lischuk S. V., Dubova E. A., Pavlov K. A., Brumberg V. A., Samoylov A. S.. "Comparison of the Therapeutic Potential of Rat and Human Mesenchymal Stromal Cells and Their Conditioned Media in Local Radiation Lesions." Medical Radiology and radiation safety 66, no. 4 (2021): 5-12. https://doi.org/10.12737/1024-6177-2021-66-4-5-12.

WIKI

{{cite journal | author = Rastorgueva A. A., Astrelina T. A., Brunchukov V. A., Usupzhanova D. Y., Kobzeva I. V., Nikitina V. A., Lischuk S. V., Dubova E. A., Pavlov K. A., Brumberg V. A., Samoylov A. S. | title = Comparison of the Therapeutic Potential of Rat and Human Mesenchymal Stromal Cells and Their Conditioned Media in Local Radiation Lesions | journal = Medical Radiology and radiation safety | year = 2021 | volume = 66 | issue = 4 | pages = 5-12 | doi = 10.12737/1024-6177-2021-66-4-5-12 }}

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COMPARISON OF THE THERAPEUTIC POTENTIAL OF RAT AND HUMAN MESENCHYMAL STROMAL CELLS AND THEIR CONDITIONED MEDIA IN LOCAL RADIATION LESIONS Comparison of the Therapeutic Potential of Rat and Human Mesenchymal Stromal Cells and Their Conditioned Media in Local Radiation Lesions

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