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Food Processing: Techniques and Technology
Journals / Food Processing: Techniques and Technology / Volume 54 Issue 3 / Effect of Biologically Active Substances on Thermal and Oxidative Stress in Caenorhabditis elegans Models
UDC 63

EFFECT OF BIOLOGICALLY ACTIVE SUBSTANCES ON THERMAL AND OXIDATIVE STRESS IN CAENORHABDITIS ELEGANS MODELS Effect of Biologically Active Substances on Thermal and Oxidative Stress in Caenorhabditis elegans Models

Published in Food Processing: Techniques and Technology · Volume 54, Issue 3 · Pages 571–584 · Rubric: ORIGINAL ARTICLE
DOI: https://doi.org/10.21603/2074-9414-2024-3-2530
Received: 26.06.2024 Accepted: 03.09.2024 Published: 02.10.2024 Language of publication: RUS
Authors
AN
Anna Frolova
ORCID SPIN
IR
Irina Milentyeva
ORCID SPIN
AN
Anastasiya Fedorova
ORCID SPIN
EK
Ekaterina Miller
ORCID
et al.
SE
Sergey Luzyanin
ORCID SPIN
ABSTRACT
Modern medicine strives to prevent age-related diseases. Oxidative stress is associated with development and progression of various diseases. Reactive oxygen species are part of vital physiological processes. High levels of reactive oxygen lead to stress and pathology whereas low ones are associated with healthy physiology. Plant-derived adaptogens demonstrate good results in stress tolerance and homeostasis. Plant materials are a pharmacologically optimal source of chemical compounds to treat various diseases, including those caused by oxidative stress. The research featured biologically active substances isolated from extracts of callus, suspension, and root cultures of medicinal plants. Baicalin and trans-cinnamic acid were obtained from Scutellaria baicalensis while ursolic acid came from Thymus vulgaris. The biologically active substances were tested for neuroprotective properties, as well as for the impact on the expression of SOD-3 and HSP-16.2. Caenorhabditis elegans served as a model to study the accumulation of carbonylated proteins and lipofuscin. The neuroprotective activity of all tested substances decreased as their concentration fell from 200 to 10 μmol/L. C. elegans proved more resistant to thermal stress if pretreated with the biologically active substances. In response to thermal stress, nematodes expressed SOD-3 more actively than HSP-16.2. At 100 μmol/L, the biologically active substances could reduce the level of carbonylated proteins. Ursolic acid was especially effective against protein carbonylation and lipofuscin accumulation in all concentrations. Baicalin, trans-cinnamic acid, and ursolic acid made it possible to reduce oxidative and thermal stress, thus demonstrating good prospects for further studies as part of adaptogenic prepa rations.
KEYWORDS
Biologically active substances, Caenorhabditis elegans, thermal stress, oxidative stress, baicalin, trans-cinnamic acid, ursolic acid
Funding
The research was part of State Assignment FZSR-2024-0008: Biologically active additives with plant metabolites against premature aging: in vitro studies. The experiments were conducted on the premises of the Applied Biotechnology Center for Collective Use, Kemerovo State University.
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